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MicroRNA (miR)-23b-3p is known to target various genes that are involved in cancer-related pathways. Exosomes are emerging intercellular communication agents. Exosomes secreted by cancer cells can deliver active molecules to the surrounding stromal cells, thereby influencing the recipient cells and promoting the development of cancers. However, the role of exosomal miR-23b-3p in salivary adenoid cystic carcinoma (SACC) is not yet clear. In this study, we set out to investigate the potential role of cancer-derived exosomal miR-23b-3p-related phosphatase and tensin homolog deleted on chromosome 10 in the alteration of angiogenesis and vascular permeability in SACC. We investigated the effect of exosomal miR-23b-3p on the progression of SACC. In vitro experiments indicated that exosomal miR-23b-3p led to an upregulation of vascular permeability, and reduced expression of tight junction proteins. In addition, exosomal miR-23b-3p also enhanced angiogenesis and migration. Next, the angiogenic effect of exosomal miR-23b-3p was validated in vivo, as it led to an increase in the tumor microvasculature. Furthermore, the growth rate of SACC was faster after injection of exosomes loaded with cholesterol-modified miR-23b-3p in mice. In conclusion, these results revealed that SACC cell-derived exosomes play an important role in promoting angiogenesis and local vascular microleakage of SACC by transporting miR-23b-3p, which suggests that miR-23b-3p in the exosomes may be a potential biomarker for distant metastasis of SACC. This suggests the potential of a novel therapeutic target by delivering anti-miR-23b-3p that focuses on exosomes.

Thirteen undescribed dimeric Erythrina alkaloids, named as erythrivarines A1-A13, were isolated from the barks of Erythrina variegata L. and. Their structures were determined on the basis of NMR, UV and mass spectral analyses. Dimeric Erythrina alkaloid with a C-8/8' linkage in erythrivarine A1 was not yet reported. Representative dimers from titled plant were used to prove their occurrence as natural products by LC - MS detection. Additionally, simultaneous investigation enabled us to propose the natural property of seemingly artificial Erythrina alkaloid with acetonyl group.

Five dimeric Erythrina alkaloids, named erythrivarines J-N, were isolated from the barks of Erythrina variegata L. (Fabaceae). The erythrivarines J-L featured a 6/6/5/6/6/5/6/6/6 ring system and super conjugated double bond systems, causing intense color from blue to wine red, while erythrivarines M-N looked orange. The structures of the isolated compounds were elucidated by 1D and 2D NMR experiments combined with MS and confirmed by the X-ray crystal diffraction technique. The performed bioassay using HEI-OC-1 cells revealed neuroprotective properties of erythrivarine N against the hearing loss causing antibiotics, neomycin.

Rural land consolidation (RLC) is beneficial to rural development to a certain extent. While acknowledging this relationship, little is known about the extent of the impact of RLC on rural vitalization at the village level and its change. Based on the theoretical analytical framework and multi-source data, this study adopts field investigation and GIS technology in Jinzhuang Village, China, to qualitatively reveal how RLC affects rural development and quantitatively analyze the effectiveness of RLC on local vitalization. The results show that greenhouse-led RLC promotes the spatial restructuring and industrial transformation of Jinzhuang Village. In this process, the restructuring of production-living-ecological space is mutually reinforced and restrictive, and compared with the decrease of the ecological space, the production space has gradually expanded and the living space has increased slightly. On the whole, RLC has boosted the development level of Jinzhuang Village. However, its main contribution lies in the socio-economic aspects, at the expense of the ecological environment, and its positive and negative effects, based on the quantitative analysis, on cultural development almost cancel each other out. Additionally, working in greenhouses for years poses a health risk to tomato farmers. Based on the above analysis and discussion, policy recommendations are provided.

Low- and middle-income countries in Southeast and East Asia face a range of challenges related to the rapid pace of urbanisation in the region, the scale of pollution, climate change, loss of ecosystem services and associated difficulties for ecological restoration. Possible pathways towards a more sustainable future lie in the applications of nature-based solutions (NBS). However, there is relatively little literature on the application of NBS in the region, particularly Southeast Asia. In this paper we address this gap by assessing the socio-ecological challenges to the application of NBS in the region – one of the most globally biodiverse. We first provide an overview and background on NBS and its underpinnings in biodiversity and ecosystem services. We then present a typology describing five unique challenges for the application of NBS in the region: (1) Characteristics of urbanisation; (2) Biophysical environmental and climatic context; (3) Environmental risks and challenges for restoration; (4) Human nature relationships and conflicts; and (5) Policy and governance context. Exploiting the opportunities through South-South and North-South collaboration to address the challenges of NBS in Southeast and East Asia needs to be a priority for government, planners and academics. Highlights Most research on Nature-Based Solutions (NBS) is in high income nations with low population growth rates and regulated urban planning.; Urban blue-green infrastructure is being degraded in low and middle-income Southeast and East Asian countries.; Applications of NBS in the region need to address the unique socio-ecological challenges.; We provide an overview of key knowledge gaps to support the implementation of NBS.

Metabolic disorders are significant in the occurrence and development of malignant tumors. Changes of specific metabolites and metabolic pathways are molecular therapeutic targets. This study aims to determine the metabolic differences between oral squamous cell carcinoma (OSCC) tissues and paired adjacent noncancerous tissues (ANT) through liquid chromatography-mass spectrometry (LC-MS). SPHK1 is a key enzyme in sphingolipid metabolism. This study also investigates the potential role of SPHK1 in OSCC.This study used LC-MS to analyze metabolic differences between OSCC tissues and paired ANT. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were applied to explain the significance of phospholipid metabolism pathways in the occurrence and development of OSCC. Through further experiments, we confirmed the oncogenic phenotypes of SPHK1 in vitro and in vivo, including proliferation, migration, and invasion.The sphingolipid metabolic pathway was significantly activated in OSCC, and the key enzyme SPHK1 was significantly upregulated in oral cancer tissues, predicting poor OSCC prognosis. In this study, SPHK1 overexpression was associated with high-grade malignancy and poor OSCC prognosis. SPHK1 targeted NF-κB by facilitating p65 expression to regulate OSCC tumor progression and promote metastasis.This study identified metabolic differences between OSCC and paired ANT, explored the carcinogenic role of overexpressed SPHK1, and revealed the association of SPHK1 with poor OSCC prognosis. SPHK1 targets NF-κB signaling by facilitating p65 expression to regulate tumor progression and promote tumor metastasis, providing potential therapeutic targets for diagnosing and treating oral tumors.

Oleanolic acid (OA, 3 β - hydroxyoleanolic acid-12-en-28-oic acid) is a pentacyclic triterpenoid present in many plants. As a new framework for development of semi synthetic triterpenoids, OA is of great significance in the discovery of anticancer drugs. Some of these derivatives, such as CDDO (2-cyano-3,12-dioxooleana-1, 9 (11)-dien-28-oic acid) have been verified in clinical trials, while other derivatives studied previously, such as SZC014, SZC015 and SZC017 (OA derivatives respectively), are also candidate drugs for cancer treatment. This paper reviews the preclinical studies, literature evidence, target analysis and anticancer mechanism of OA and its derivatives. The mechanism of action of its derivatives mainly includes anti-cancer cell proliferation, inducing tumor cell apoptosis, inducing autophagy, regulating cell cycle regulatory proteins, inhibiting vascular endothelial growth, anti angiogenesis, inhibiting tumor cell migration and invasion. In recent years, the molecular mechanism of OA and its derivatives has been elucidated. These effects seem to be mediated by the alterations in a variety of signaling pathways induced by OA and its derivatives. In conclusion, OA and its derivatives are considered as important candidate drugs for the treatment of cancer, indicating that OA and its derivatives have the potential to be used as anticancer drugs in practice.

Studies have shown the inhibitory effect of microRNA-34a on proliferation, migration, and invasion of oral squamous cell carcinoma. However, the lack of a safe and effective delivery system limits the clinical application of microRNA-34a in oral cancer treatment. An exosome is a small extracellular vesicle that mediates intercellular communication by delivering proteins, nucleic acids, and other contents, and functions as a natural drug delivery carrier. Here, we aimed to explore whether exosomes could be used to load microRNA-34a via co-incubation and further used to treat OSCC. Ultracentrifugation was used to obtain exosomes derived from HEK293T cells and the extracted exosomes were analyzed via transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Subsequently, we loaded cholesterol-modified microRNA-34a into HEK293T cell exosomes by co-incubation. Then, PKH67 and Cy3 co-labeled exo-microRNA-34a were co-incubated with HN6 cells and exosome entry into the HN6 cells was observed using a confocal laser scanning microscope. The cell proliferation, migration, and invasion were assessed by CCK-8 and Transwell assay analysis. SATB2 expression in HN6 cells was analyzed via western blotting. In this study, cholesterol-modified microRNA-34a was loaded into exosomes of HEK293T cells by co-incubation. The microRNA-34a-loaded exosomes were secreted from HEK293T cells and were absorbed by HN6 oral squamous carcinoma cells. Further, microRNA-34a-loaded exosomes led to a significant inhibition of HN6 cell proliferation, migration, and invasion by down regulating SATB2 expression. These results report a new delivery method for microRNA-34a, providing a new approach for the treatment of oral cancer.

BACKGROUND miRNAs and mRNAs have been significantly implicated in tumorigenesis and served as promising prognostic biomarkers for human cancer. Hence, this study was aimed to develop the pivotal miRNA biomarkers-based prognostic signature for salivary adenoid cystic carcinoma. METHODS The miRNA and mRNA expression data were integrated from the gene expression omnibus database to study their involvement in salivary adenoid cystic carcinoma development and progression. Gene ontology and kyoto encyclopedia of genes and genomes were conducted to analyze the biological pathways. Reverse transcription-quantitative PCR was used to verify the expression of selected miRNAs in salivary adenoid cystic carcinoma and corresponding normal tissues. RESULTS There were 386 differentially expressed genes: 158 upregulated and 228 downregulated genes and 102 differentially expressed miRNAs: 78 upregulated and 24 downregulated miRNAs in the salivary adenoid cystic carcinoma samples. A miRNA-mRNA network containing 11 miRNAs and 199 genes was subsequently constructed. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis revealed that the genes targeted by the 11 miRNAs were mostly involved in tumor-related pathways and processes, such as miRNAs in cancer, focal adhesion, neurotrophin signaling pathway, and the PI3K-Akt signaling pathway. Among them, 4 miRNAs (miR-375, miR-494, miR-34c-5p, and miR-331-3p) were selected to verify by reverse transcription-quantitative PCR in 36 pairs of collected salivary adenoid cystic carcinoma and adjacent nontumor samples. Overall survival analysis revealed that the higher expression of miR-331-3p was significantly associated with a worst overall survival and multivariate Cox regression analysis suggested that hsa-miR-331-3p could be an independent prognostic factor for salivary adenoid cystic carcinoma. CONCLUSION Our results revealed that 4-miRNAs signature was a powerful prognostic biomarker for salivary adenoid cystic carcinoma, which provide a basis for exploring deeper mechanisms regarding the progression of salivary adenoid cystic carcinoma, and leading to the development of potential therapeutic strategies.

Five dimeric

Sustainable municipal solid waste (MSW) management is regarded as one of the key elements for achieving urban sustainability via mitigating global climate change, recycling resources and recovering energy. Landfill is considered as the least preferable disposal method and the EU Landfill Directive (ELD) announced in 1999 requires member countries to reduce the volume of landfilled biodegradable materials. The enforcement of ELD initiated the evolution of MSW management system UK. This study depicted and assessed the transition and performance of MSW management after the millennium in Nottingham via materials flow analysis (MFA), as well as appropriately selected indicators based on the concept of waste management hierarchy and targets set in waste management regulations. We observed improvements in waste reduction, material recycling, energy recovery, and landfill prevention. During the period 2001/02 to 2016/17, annual waste generation reduced from 463 kg/Ca to 361 kg/Ca, the recycling and composting share increased from 4.6% to 44.4%, and the landfill share reduced from 54.7% to 7.3%. These signs of progress are believed to be driven by the ELD and the associated policies and waste management targets established at the national and local levels. An alternative scenario with food waste and textile separation at source and utilizing anaerobic digestion to treat separately collected organic waste is proposed at the end of this paper to fulfil the high targets set by local government and we further suggest that the recycling share may be improved by educating and supporting the public on waste separation at the sources.

Ten dimeric and two monomeric Erythrina alkaloids, all of them are undescribed, were isolated from the bark of Erythrina variegata L. and named as erythrivarines O-Z. Their structures were determined on the basis of NMR and UV-spectroscopy and mass spectrometry. Dimeric Erythrina alkaloids with a C-10/11' linkage in erythrivarine O and a C-7/10' connectivity in erythrivarines P-U are not yet reported. The two identified monomeric alkaloids may be the precursors of the described dimeric derivatives. These co-occurring dimeric and monomeric alkaloids enabled us to propose a possible biosynthetic pathway leading to these dimers. Their effects of preventing hearing loss were additionally evaluated and erythrivarine T showed as a potential protector of the House Ear Institute-Organ of Corti 1 (HEI-OC-1) cells against neomycin.

The current study aimed to explore the effect of Follistatin-like 1 (FSTL1) on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in an inflammatory environment.Animal models of FSTL1-deficiency and wild-type mice were used, and the micro-CT images of the femoral head were evaluated. Mouse bone marrow mesenchymal stem cells were treated with various concentrations of recombinant FSTL1 (rFSTL1) in an inflammatory environment in vitro. Meanwhile, overexpression or knockdown of FSTL1 through lentiviral transfection was performed. Alkaline phosphatase (ALP) activity was tested, and Alizarin Red staining (ARS) was performed to evaluate osteogenic differentiation ability. The mRNA expression level of osteogenesis-related genes was detected by RT-qPCR.In vivo experiments revealed a higher number of femoral skulls, higher trabecular thickness, smaller trabecular space, and less osteoporosis in FSTL1-knockdown mice than in the wild-type mice. The BMSCs with overexpression of FSTL1 or those treated with recombinant FSTL1 (rFSTL1) showed suppression of ALP activity, calcium nodule formation, and expression of osteogenesis-related genes osteopontin (OPN), osteocalcin (OCN), collagen type I alpha 1 (Col1α1), and more importantly, rFSTL1 functions in a dose-dependent manner. In contrast, FSTL1 knockdown promoted the osteogenesis activity and the expression of these osteogenesis-related genes in vitro.FSTL1 is an osteogenic suppressor that inhibits the osteogenic differentiation of BMSCs during inflammation and it can be a new target for bone regeneration.

Environmentally persistent free radicals (EPFRs) are a new class of pollutants that are long-lived in fine particles (PM(2.5)), i.e., their 1/e lifetime ranges from days to months (or even infinite). They are capable of producing harmful reactive oxygen species such as hydroxyl radicals. The redox cycling of EPFRs is considered as an important pathway for PM(2.5) to induce oxidative stress inside the humans, causing adverse health effects such as respiratory and cardiovascular diseases. Consequently, research regarding their toxicity, formation and environmental occurrences in PM(2.5) has attracted increasing attentions globally during the past two decades. However, literature data in this field remain quite limited and discrete. Hence, an extensive review is urgently needed to summarize the current understanding of this topic. In this work, we systematically reviewed the analytical methods and environmental occurrences, e.g., types, concentrations, and decay behaviors, as well as possible sources of EPFRs in PM(2.5). The types of pretreatment methods, g-values of common EPFRs and categories of decay processes were discussed in detail. Moreover, great efforts were made to revisit the original data of the published works of EPFRs in airborne particulate matter and provided additional useful information for comparison where possible, e.g., their mean and standard deviation of g-values, line widths (ΔH(p-p)), and concentrations. Finally, possible research opportunities were highlighted to further advance our knowledge of this emerging issue.

Two new dinuclear lanthanide complexes, namely, [Ln2L(dbm)4]·2CH3OH (Ln = Gd (1) and Dy (2), H2L = N,N′-bis(3-methoxysalicylidene)-1,3-diamino-2-propanol, Hdbm = 1,3-diphenyl-1,3-propanedione) have been synthesized and characterized by elemental analyses, single crystal X-ray diffraction and magnetic measurements. The studies of single crystal X-ray diffraction analysis demonstrate that both 1 and 2 are isostructural with a [Ln2] core. Magnetic investigations reveal the presence of a weak antiferromagnetic interaction in 1 and 2, respectively, of which 1 displays magnetocaloric effect with a large magnetic entropy change (−ΔSm = 18.95(2) J kg−1 K−1 for ΔH = 7 T at 2 K), while 2 exhibits slow relaxation of magnetization with the anisotropy energy barrier ΔE/kB = 0.73(1) K.

Arsenic (As) pollution remains a major threat to the quality of global soils and drinking water. The health effects of As pollution are often severe and have been largely reported across Asia and South America. This study investigated the possibility of using unmodified biochar derived from rice husk (RB) and aspen wood (WB) at 400 °C and 700 °C to enhance the precipitation of calcium/arsenic compounds for the removal of As(III) from solution. The approach was based on utilizing calcium to precipitate arsenic in solution and adding unmodified biochar to enhance the process. Using this approach, As(III) concentration in aqueous solution decreased by 58.1% when biochar was added, compared to 25.4% in the absence of biochar. Varying the pH from acidic to alkaline enabled an investigation into the pH dependent dynamics of the approach. Results indicated that significant precipitation was only possible at near neutral pH (i.e. pH = 6.5) where calcium arsenites (i.e. Ca(AsO2)2, and CaAsO2OH•½H2O) and arsenates (i.e. Ca5(AsO4)3OH) were precipitated and deposited as aggregates in the pores of biochars. Arsenite was only slightly precipitated under acidic conditions (pH = 4.5) while no arsenite was precipitated under alkaline conditions (pH = 9.5). Arsenite desorption from wood biochar was lowest at pH 6.5 indicating that wood biochar was able to retain a large quantity of the precipitates formed at pH 6.5 compared to pH 4.5 and pH 9.5. Given that the removal of As(III) from solution is often challenging and that biochar modification invites additional cost, the study demonstrated that low cost unmodified biochar can be effective in enhancing the removal of As(III) from the environment through Ca–As precipitation.

Polycyclic aromatic hydrocarbons (PAHs) have gained attention because of their environmental persistence and effects on ecosystems, animals, and human health. They are mutagenic, carcinogenic, and teratogenic. The review provides background knowledge about their sources, metabolism, temporal variations, and size distribution in atmospheric particulate matter. The review article briefly discusses the analytical methods suitable for the extraction, characterization, and quantification of nonpolar and polar PAHs, addressing the challenges. Herein, we discussed the molecular diagnostic ratios (DRs), stable carbon isotopic analysis (SCIA), and receptor models, with much emphasis on the positive matrix factorization (PMF) model, for apportioning PAH sources. Among which, DRs and PCA identified as the most widely employed method, but their accuracy for PAH source identification has received global criticism. Therefore, the review recommends compound-specific isotopic analysis (CSIA) and PMF as the best alternative methods to provide detailed qualitative and quantitative source analysis. The compound-specific isotopic signatures are not affected by environmental degradation and are considered promising for apportioning PAH sources. However, isotopic fractions of co-eluted compounds like polar PAHs and aliphatic hydrocarbons make the PAHs isotopic fractions interpretation difficult. The interference of unresolved complex mixtures is a limitation to the application of CSIA for PAH source apportionment. Hence, for CSIA to further support PAH source apportionment, fast and cost-effective purification techniques with no isotopic fractionation effects are highly desirable. The present review explains the concept of stable carbon isotopic analysis (SCIA) relevant to PAH source analysis, identifying the techniques suitable for sample extract purification. We demonstrate how the source apportioned PAHs can be applied in assessing the health risk of PAHs using the incremental lifetime cancer risk (ILCR) model, and in doing so, we identify the key factors that could undermine the accuracy of the ILCR and research gaps that need further investigation.

Delineating the margins of Oral squamous cell carcinoma (OSCC) is a critical step for optimaltumor resection. The aim of this study was to evaluate the accuracy of lesion surgical margin identification using autofluorescence visualization.Thirty patients with OSCC were included in this study. For each lesion, the fluorescence loss boundary was determined using VELscope before ablative surgical resection (with a 1.5-2cm safety margin) was performed. A total of 126 samples were obtained from 30 surgical specimens, each containing the tissue from the fluorescence loss boundary to surgical margin. The status of each sample was determined by oral pathologists and the staining intensities of Ki-67, E-cadherin, and Vimentin at the fluorescence loss boundary and surgical margin were evaluated by immunohistochemistry.Fluorescence loss regions were identified in all patients. Of the 126 samples collected, HE staining identified 77 normal epithelia (61.1%), 26 mild dysplasia (20.6%), 17 severe dysplasia (13.4%) and 6 carcinomas in situ (4.9%). A significant correlation was found between the differentiation grade of tumor cells and the pathological status of the surgical marginal specimens (P0.05). Forty-two of the 126 samples were randomly selected for further immunohistochemical staining. No significant differences were seen in Ki-67, E-cadherin, or Vimentin expression at the fluorescence loss boundary or surgical margin, however, the proteins' expression level was positively correlated with the degree of dysplasia (P0.01).Autofluorescence visualization has potential as a simple surgical margin setting device for OSCC and may help delineate the superficial area of OSCC with acceptable accuracy. However, when considering the inherent limitations of this system, we suggest that the approach should only be applied under certain conditions, such as when dealing with superficial, well-differentiated lesions.

The Urban Heat Island (UHI) effect occurs when the temperature in an urban area is higher than the temperature in a rural area. UHIs are generally monitored using remote sensing techniques such as satellite imagery or using temperature sensors deployed in a metropolitan area. In this chapter, a newly proposed methodology to monitor the UHI intensity from Global Navigation Satellite Systems (GNSS) data is described. As the GNSS signal travels from the satellite to the receiver it propagates through the troposphere the travelling signal is delayed by the troposphere. The tropospheric delay is proportional to environmental variables. The tropospheric delay in zenith direction (ZTD) is estimated as part of the Precise Point Positioning (PPP) technique. Therefore, this chapter shows how to process GNSS data to obtain ZTD and how to obtain temperature at an urban and a rural site simultaneously from the ZTD. The advantages of using GNSS data is its availability and many GNSS networks have been deployed in different cities so no need to deploy sensor networks. Furthermore, GNSS signal is less affected by bad weather conditions than satellite imagery.

Faced with the challenges to deal with increasingly growing and ever diversified municipal solid waste (MSW), a series of waste directives have been published by European Commission to divert MSW from landfills to more sustainable management options. The presented study assessed the transition of MSW man-agement in Nottingham, UK, since the enforcement of the EU Landfill Directive using a tool of combined materials flow analysis (MFA) and life cycle assess-ment (LCA). The results show that the MSW management system in Nottingham changed from a relatively simple landfill & energy from waste (EfW) mode to a complex, multi-technology mode. Improvements in waste reduction, material re-cycling, energy recovery, and landfill prevention have been made. As a positive result, the global warming potential (GWP) of the MSW management system re-duced from 1,076.0 kg CO2–eq./t of MSW in 2001/02 to 211.3 kg CO2–eq./t of MSW in 2016/17. Based on the results of MFA and LCA, recommendations on separating food waste and textile at source and updating treatment technologies are made for future improvement.

Veterinary antibiotics are intensively and widely used in animal farming to treat or prevent diseases, as well as improve growth rate and feed efficiency. Animal manure is an important reservoir of veterinary antibiotics due to their high excretion rates, and thus manure application has been a critical source of veterinary antibiotics in agro-ecosystems. However, how veterinary antibiotics affect agroecosystem functions is still unclearly understood. In this study, we evaluated the effects of veterinary antibiotics on soil bacteria and earthworms in agricultural land with long-term manure application. The potential mechanisms of antibiotic-induced changes in crop yields were also revealed. The results showed that the increasing prevalence of veterinary antibiotics in agro-ecosystems inhibited earthworm abundance and bacterial diversity, and then decreased the bioavailability of soil nutrients. Furthermore, high-dose exposure to veterinary antibiotics improved the abundance of plant pathogenic bacteria. Analysis indicated that veterinary antibiotics played an important underlying role in driving the negative effects on peanut grain yields via disturbing microbe- and earthworm-mediated soil available nutrient contents. The direct toxicity effects of antibiotics on peanut relative yields were stronger than their indirect mediating effects. Additionally, the tradeoffs between antibiotics and agroecosystem functions increased at low exposure levels and then decreased at high exposure levels, which indicated the effects of antibiotics on agroecosystem functions were dose-dependent, except for earthworm biomass. Antibiotic contamination will impose threats to agricultural sustainability was highlighted and should be paid more attention.

European Commission has promulgated a series of directives to divert MSW from landfills to more sustainable management options. To investigate whether the directives affect the MSW management practice locally, the transition of MSW management in Nottingham, UK, since the enforcement of the EU Landfill Directive, has been assessed by combining materials flow analysis (MFA) and life cycle assessment (LCA). The results confirm that paralleled to the enforcement of the EU directives MSW management system in Nottingham evolved from a simple landfill and energy from waste (EfW) mode to a complex multi-technology mode. Landfill rate has been reduced through waste prevention, material recycling, and energy recovery. The complex multi-technology mode contributed to the reduced GHG emission per unit waste treated from 1,076.0 kg CO2–eq./t in 2001/02 to 228.1 kg CO2–eq./t in 2019/20. The temporal correlation between enforcement of EU directives and the progression of the MSW management in Nottingham depicted a trickle-down effect of the directives driving the improvement of local MSW management. Similar temporal patterns were exhibited among the EU member states. Interestingly, some European countries whose landfill rate already surpass the requirement prior to the enforcement of the related EU directives continued advancing waste management system, mostly incentivized by extracting valuable materials or energy from the well-sorted waste. This incentive, aligning with the principle of circular economy, may become the future drive of cities like Nottingham, who either achieved the regulatory targets or without regulatory incentives by the international governmental bodies.

Land consolidation (LC), as an effective instrument to relieve rural land issues and promote rural sustainability, has already attracted the attention of the Chinese government and academia. Some Chinese scholars have proposed strategies to promote the development of LC by investigating cases in European countries and comparing them with that in China. However, the introduction of LC in Europe as a whole and China has not been done systematically from a comparative perspective. This paper aims to offer a broader view of the development of LC in China and Europe and identify points for improvement based on the current Chinese political system and European experience. Given the special sociocultural background of Europe, it has been divided into Central, Eastern Europe, and Western Europe in the comparison and analysis. The comparison of LC, therefore, was conducted from the aspects of historical background, political will, legislation, procedure, and public participation. This is the first attempt to compare LC between Europe as a whole and China in literature. It identifies reasons behind the differences between China and Europe, and considers possible lessons that China might learn from the experience of LC in Europe. Based on these, the paper puts forward some countermeasures and suggestions for the development of LC in China.

Surface water flooding is currently viewed as the most serious water-related issue in many of the China’s large cities due to rapid urbanization, land-use change and the process of rapid socio-economic development. In 2014, the People’s Republic of China established the concept of the ‘Sponge City’, which will be used to tackle urban surface-water flooding and related urban water management issues, such as purification of urban runoff, attenuation of peak run-off and water conservation. The concept is being developed to make use of ‘blue’ and ‘green’ spaces in the urban environment for stormwater management and control. It is envisaged that related practices will enhance natural ecosystems and provide more aesthetically pleasing space for the people that live and work in urban environments, in addition enabling nature-based solutions to improve urban habitats for birds and other organisms. Until recently, grey infrastructure and hard engineering-based management approaches have been adopted in the rapidly developing Chinese urban environment as urban flood and drainage issues are predominantly managed by municipal water engineers. The Sponge City concept and related guidelines and practices will provide multiple opportunities to integrate ideas from eco-hydrology, climate change impact assessment and planning, and consideration of long-term social and environmental well-being, within the urban land-use planning process. This paper aims to explicate the Sponge city concept and its development, and consider the implications of the transformation of urban land-use planning and urban-water management practice in China. To achieve the dual goals of sustainable water-use and better flood control (as targeted by the Sponge City concept), more effective development and implementation of land-use guidance and assessment tools (with explicit integration of urban flood-risk assessment, land-drainage guidance, climate projection methods, and assessment of long-term sustainability) are recommended.

The Urban Heat Island (UHI) effect occurs when an urban area experiences higher temperatures than its rural surrounding because of heat being absorbed by built structures and heat being released by anthropogenic sources. UHIs can cause adverse effects to human health and increase energy consumption used for cooling buildings. Therefore, it is important to monitor accurately the UHI effect. The intensity of UHIs are usually monitored using satellite imagery, airborne sensors, and surface temperature sensors. Satellite imagery can cover a large area but requires a clear sky to obtain good images. Moreover, airborne sensors are expensive and also require a clear sky to obtain good data. A large network of surface temperature sensors is required to monitor the UHI of an entire region, which can also be expensive. In this paper, we present a three-step algorithm to monitor UHI intensity using data from Global Navigation Satellite Systems (GNSS). The advantages of using GNSS data to monitor the UHI effect are the increased availability of observation data, high temporal resolution and high geographical resolution. The first step of the algorithm is the calculation of a priori environmental parameters (i.e., water vapour partial pressure, troposphere height, surface pressure, and the vertical profile of refractivity) from radiosonde data. The second step is the calculation of temperature from GNSS data. The last step is the UHI intensity computation. The algorithm presented in this paper has been tested and validated using publicly available GNSS and meteorological data from Los Angeles, California, USA. The validation of the algorithm is done by comparing the UHI intensity estimated from the algorithm with temperature data obtained from weather stations. In the validation, the proposed algorithm can achieve an accuracy of 1.71 °C at 95 % confidence level.

As human society has developed, food production has developed into a transboundary supply chain that produces wastes during each stage. Decomposed food wastes may release greenhouse gases that contribute to the warming effects of the climate; if incinerated, they could also become sources of SO2 and NOx air emissions, which mix with the precipitation and, thus, acidify the soil and water. Degraded and soluble leachate from the food residuals, abundant in nitrogen and phosphorus, may facilitate eutrophication of surface and groundwater. In this chapter, the added impacts on the climate and water resources in relation to food waste are evaluated based on carbon and water footprints. The results showed that the production of livestock, in general, produced a higher carbon footprint than that of plant-based food. On the other hand, the production of cereals, in particular, rice production, contributed to a large amount of total water footprint due to the need of irrigation. Using newly developed treatment technologies, food waste can be a good source for resources and energy recovery that offsets the carbon emission. However, the uncontrolled or contained leachate from food waste treatment can still cost a considerable amount of gray water footprint. In terms of the waste produced during food production, developing countries contributed greater footprints for less-than perfect agricultural practices and waste treatments. They are, however, the major food producers worldwide. On the other hand, developed countries are larger contributors to the footprints during the consumption stages of the food supply chain. Consumers in these countries tend to dispose large quantities of undesirable food after purchasing. To reduce food waste, it may be mutually beneficial for developed countries to export useful technologies in terms of agricultural practices and waste treatments to developing countries. For waste reduction after the food is purchased, education and policy that incentivize consumers to reduce food waste may be the key.

Seafood is an important exposure route for mercury, especially methyl mercury (MeHg). Therefore, we quantified MeHg concentrations in 69 species of seafood including fish, crustaceans and mollusks collected from Zhoushan Islands, China. MeHg concentrations ranged from 1. The daily dietary intake and hazard quotient for MeHg were calculated to estimate exposure and health risk through seafood consumption by local inhabitants. The calculated HQ was lower than 1, thus indicating that the exposure was below the risk threshold of related chronic diseases. However, higher MeHg concentrations in fish species such as Scoliodon sorrakowah and Auxis thazard are concerning and may pose health risk through continuous consumption by local inhabitants. China Spark Program (2015GA700094); Medical Health Science Foundation Program of the Health Department of Zhejiang Province (2020RC137); Science and technology Program of Zhoushan City (2017C32089); Medical Health Science Foundation Program of the Health Department of Zhoushan City (2018G02)) and the Chinese Academy of Sciences Fellowships under the Chinese Academy of Sciences President's International Fellowship for Visiting Scientists (2018VCC0002). info:eu-repo/semantics/publishedVersion

Ningbo is a coastal megacity located at the East Coast of China and developing rapidly with proactive trading and export economic activities. The city owns a ranked top ten international port and it is the major strategic spot of 21st century maritime Silk Road from the “Belt and Road” policy established for promoting further international trades and developments. In future, populations and economy in Ningbo are expected continuously growing in the next few decades. The demand of quality freshwater resources thus is enormously increasing. Ningbo municipal government has established the “Five water management” (五水共治) policy in 2013 that aims to manage (i) sewage discharge; (ii) flooding; (iii) surface water; (iv) water conservation and (v) freshwater supply. Indeed, the municipal government also liaised and initiated the “Sponge City Program” after 2015 that Ningbo was selected as one of the pilot city; these policies and practices are successful up to now. This article adopts the case study of Ningbo to investigate the reasons of municipal government to promote the policy, to understand the public perception of this water management policy in Ningbo through conducted semi-structured interviews. During the 2017 and 2019, we conducted a questionnaire (N = 110) and interviews (N = 10) that follow up for justification of the public perception with the local communities. Our findings indicated that the communities had not been engaged closely with these practices, but generally supporting these two urban water management practices; and agreed that the urban water conditions (urban floods and pollution) had been improved. Also, the article discusses whether these (5 Water and SCP) practices can be extensively applied in other Chinese cities. We will provide recommendations at the end of the article.

As an important part of the terrestrial ecosystem, agriculture ecosystem plays a key role in supporting and maintaining human survival on earth. To restore contaminated sites as well as improve soil fertility, applying biochar to soil is an environmentally approachable technology. To evaluate the benefit of applying peanut shells biochar (PNB) to the soil, a glasshouse experiment was conducted at a ratio of biochar of 2%, 4%, and 6% of either type of biochar. The results indicated that PNB application increased soil pH, electric conductivity (EC), total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) in soil. The bioavailable concentration of PTEs such as cadmium (Cd), nickel (Ni), arsenic (As), chromium (Cr) and lead (Pb) significantly (P ≤ 0.05) decreased by about 25%, 50%, 31%, 55%, and 41% by applying PNB at ratio of 6%. Furthermore, in the same treatment group (PNB-6%), the significant (P ≤ 0.05) by about 68%, 55%, and 38% respectively. A statistically highest significant (P ≤ 0.05) increase in concentration of chlorophyll a, b (125%, 350%) and carotenoid (166%) was found in the B. oleracea shoots treated with the highest ratio of PNB (6%). Leaf soluble protein significantly (P ≤ 0.05) declined by about 40%, 60%, and 80% as well as 30%, 50%, and 70%. The results suggested that PNB-6% was the best acidic soil amendment among the tested treatment. However, larger-scale studies are needed to verify the effects of PNB on plant growth, toxicity, and soil properties in diverse environmental conditions before widespread agricultural application.

The current study assesses the persistent organic pollutants (POPs) in water and their bioaccumulation in Brown Trout (BT) and Rainbow Trout (RT) present in the remote alpine lakes (RALs) of Himalayas, Pakistan. Hence, these environments might receive POPs by long range atmospheric transport (LRAT) with little interferences from local anthropogenic activities. The potential transportation pathways for such accumulation can be atmospheric precipitation, melting ice and glacial runoff. The results indicated that the sum of mean concentrations of organochlorine pesticides (OCPs (DDTs + HCHs)) in water of Glacial-fed lakes, Ice melting-fed lake and Rain-fed lake ranged from 0.21 to 317, 0.14-293, 0.16-235 pg L-1, respectively, while those of polychlorinated biphenyls (PCBs) are 0.275-16.02, 0-15.88, 0.234-14.46 pg L-1, respectively. Similarly, the sum of mean concentrations of all investigated PCBs and OCPs in BT ranged from 0.008 to 0.715 ng g-1 and 0.003-3.835 ng g-1 based on wet weight (ww), while for RT these concentrations were 0.002-0.557 ng g-1 and 0.001-1.402 ng g-1 (ww), respectively in the selected RALs. The PCBs and OCPs levels in both water and fish tissues were observed in order of Glacial-fed lakes > Ice melting-fed lake > Rain-fed lake. The results proposed that melting of ice and glacial water are more significant sources of the contaminants (PCBs and OCPs) in the freshwaters of RALs of Himalayas as compared to rainfall water. The positive correlation between water contamination and the selected fish species confirmed that these can be used as a bio-indicator for future research studies. However, the impact of POPs as they cascade through downstream ecosystems remains largely unexplored. The additional study of contaminant dynamics should be extended to a wide range of mountain environments of Himalayas, where melt-water used for drinking and irrigation purposes by billions of people reside alone the waterways that originated from these remote areas.